Abstract Details 145

Reconstruction of Difference Electron Density in a Pump-probe Experiment on an Ensemble of Biomolecules:Effects of Poisson Noise
Abstract ID 145
Presenter Kanupriya Pande
Presentation Type Poster
Full Author List Marius Schmidt, Dilano Saldin

University of Wisconsin Milwaukee


We describe a method for reconstructing the time-resolved difference electron density in a pump-probe experiment by analyzing x-ray intensities scattered from an ensemble of identical biomolecules. The method relies on measuring the angular correlations of intensities, averaged over a large number of diffractions patterns from randomly oriented particles. The differences between intensity correlations from the dark and photoexcited structures enable the direct calculation of the difference electron density with a prior knowledge of the dark structure only. We consider the limitations imposed by Poisson noise and on the number of particles per diffraction snapshot on the application of the proposed algorithm for structure recovery.



Funding Acknowledgement